dnase max kit (discontinued) (Qiagen)

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Qiagen dnase max kit (discontinued)
Dnase Max Kit (Discontinued), supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnase+max+kit/rneasy+plus+mini+kit/pmc10086458__gutjnl___2022___327166supp001-226-106-117
Average 90 stars, based on 1 article reviews

dnase max kit (discontinued) - by Bioz Stars, 2026-10

90/100 stars

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Article Title: Biochar@MIL-88A(Fe) accelerates direct interspecies electron transfer and hydrogen transfer in waste activated sludge anaerobic digestion: Exploring electron transfer and biomolecular mechanisms.
Article Snippet: Adding additives exogenously is an effective strategy to enhance methanogenic activity and improve AD stability.. Corn straw-based biochar@MIL-88A(Fe) (BM) was synthesized herewith and used as an exogenous additive to boost methane (CH4) production.. After adding BM at 250 mg/g WAS VS, the accumulative CH4 production and maximum CH4 yield increased by 1.2 and 1.9 times, respectively, with CH4 comprising 88% of the biogas.

Article Title: Metatranscriptomic responses of High-Arctic tundra soil microbiomes to carbon input
Article Snippet: RNA samples were cleaned with the RNeasy PowerClean Pro CleanUp Kit (Qiagen, Hilden, Germany), and any remaining DNA contamination was subsequently removed using the DNase Max Kit (Qiagen).

Article Title: Circulating miR-199a and long noncoding-RNA ANRIL as Promising Diagnostic Biomarkers for Inflammatory Bowel Disease.
Article Snippet: From the *Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt †Department of Microbiology and Immunology, Faculty of Pharmacy, Fayoum University, Fayoum, Egypt ‡Department of Tropical Medicine, Faculty of Medicine, Fayoum University, Fayoum, Egypt §Department of General Medicine, Faculty of Medicine, Fayoum University, Fayoum, Egypt ¶Department of Medical Microbiology and Immunology, Faculty of Medicine, Cairo University, Cairo, Egypt ∥Department of Clinical Pathology, Faculty of Medicine, Damietta University, Damietta, Egypt **Department of Rheumatology, Faculty of Medicine, Fayoum University, Fayoum, Egypt ††Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt ‡‡Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Fayoum University, Fayoum, Egypt Address correspondence to: Mahmoud A.F.. Khalil, PhD, Fayoum University, Fayoum, EGYPT (maf04@fayoum.edu.eg).

Article Title: Antibiotic resistomes and ecological risk elimination in field-scale constructed wetland revealed by integrated metagenomics and metatranscriptomics.
Article Snippet: • A systematic metagenomic and metatranscriptomic survey of the field-scale

Reverse Transcription:

Article Title: IL-20 subfamily cytokines impair the esophageal epithelial barrier by diminishing filaggrins in eosinophilic esophagitis
Article Snippet: RNA was extracted from human esophageal biopsies, mouse esophagus, bone marrowderived macrophages, and the KYSE-180 cell line using TRI Reagent (Sigma-Aldrich) or Direct-zol RNA MiniPrep (Zymo Research) according to the manufacturer’s instructions. .. After removing DNA contaminants with DNase Max Kit (Qiagen), RNA samples were reverse transcribed using High Capacity cDNA Reverse Transcription (Applied Biosystems) according to the manufacturer’s instructions. .. The SYBR Green PCR (Qiagen) or TakyonLow Rox SYBR MasterMix blue (Eurogentec) kits were used for the quantitative PCR, and PCR was run on an ABI ViiA 7 cycler.

Centrifugation:

Article Title: LncRNA NEAT1 and miRNA 101 as potential diagnostic biomarkers in patients with alopecia areata
Article Snippet: .. The column was transferred into a new 1.5-mL collecting tube and subjected to centrifugation for 2 min at 8000× g . The RNA was subsequently eluted by directly pipetting RNase-free water (50 μl) onto the column and centrifuging it at 8000× g for 1 min. After the sample was extracted, any residual DNA was removed using DNase, and then the DNase Max Kit was utilized (Qiagen, Valencia, CA, USA) to reverse-transcribe it into cDNA. .. The RNA was measured using a NanoDrop 2000 (Thermo Scientific, Waltham, MA, USA) spectrophotometer at 260/280 nm.

Purification:

Article Title: Effect of Corticosterone on Gene Expression in the Context of Global Hippocampal Transcription.
Article Snippet: Total RNA was extracted from 60 individual hippocampal samples (10 in each group, Figure 1) using the GeneMATRIX Universal RNA Purification Kit (Eurx, Gdańsk, Poland) according to the manufacturer-supplied protocol. .. The RNA samples obtained were additionally purified with the DNase Max kit (Qiagen) to remove remnants of DNA. .. The quantity and quality of RNA samples were assessed by spectrophotometry (ND-1000, Nanodrop) and microcapillary electrophoresis (Bioanalyzer 2000, Thermo Fisher Scientific, Waltham, MA, USA).

Sequencing:

Article Title: Small sample amounts from rhizosphere of barley maintain microbial community structure and diversity revealed by total RNA sequencing.
Article Snippet: Illumina library Preparation and sequencing We prepared libraries for subsequent sequencing using the reagents and procedures described by Mondini et al. [26]. .. Prior to constructing sequencing libraries, we treated the RNA with DNAse Max kit (QIAGEN) to eliminate residual DNA that may have co-eluted with the RNA. .. Libraries were prepared utilizing the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) in conjunction with the NEBNext MultiPlex Oligos for Illumina Set 1 (New England Biolabs), following the manufacturer’s protocol with the following specifications: no fragmentation, 25-fold adapter dilution, and 13 cycles of amplification.

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